Catalogue No.
15090203
OX-137
OX-137
Cell Line Name
OX-137
Cell Line Description
The hybridoma cell line OX-137 secretes mouse monoclonal IgG1 antibodies reactive with human SIRPβ1 membrane protein.
General Info
Fusion Species
Balb/C mouse
Target (Reactivity)
Human SIRPβ2
Also known as
OX137, OX-137
Unique to ECACC
Yes
Release Conditions
Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.
Characteristics
Myeloma
Sp2/0-Ag14
Immunogen
Recombinant protein
Antibody Isotype
IgG1
Morphology
Spherical
Tissue of Origin
Hybrid
Culture Conditions
Cell Type
Hybridoma
Subculture Routine
Seed at 5x104 cells/ml. Subculture when cells reach ~5x105 cells/ml (usually after 3-4 days); 5% CO2; 37°C
When recovering hybridoma cultures from frozen it is not unusual for growth to be slower than expected initially and there may be an observed decrease in viability. Establishment of an actively proliferating culture may take up to 2 weeks.
On resuscitation a centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media+. Remove a sample for counting. Centrifuge at 100 x g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 105 cells/ml. Place culture flask flat and observe regularly until viable proliferating cells are seen.
Culture Medium
RPMI 1640 + 4mM glutamine + 10% Foetal Bovine Serum (FBS)
Often hybridoma cultures may benefit from being resuspended with media supplemented with 20% FBS in the early critical stage of culture establishment immediately post resuscitation.
Growth Mode
Suspension
Additional Info
Depositor
Deposited by: LifeArc (MRC Technology), 7th Floor Lynton House, 7-12 Tavistock Square, London, WC1H 9LT Originator: Neil Barclay, Sir William Dunn School of Pathology, University of Oxford, OX1 3RE
Country of Origin
United Kingdom
Applications
Recognises human SIRPβ2 but not SIRPα or SIRPβ1
Patent
None specified by the Depositor
GMO Status
Not Applicable
Hazard Group (ACDP)
2
Available Formats
- Frozen
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: OX-137 (ECACC 15090203)
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.