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Catalogue No.


Cell Line Name


Cell Line Description

The hybridoma cell line OX-133 secretes mouse monoclonal IgG antibodies reactive with N-ethyl maleimide (NEM) modified proteins

General Info

Fusion Species

Mouse Sp2/0-Ag14

Also known as


Unique to ECACC


Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.



Recombinant protein reduced and then alkylated with N-ethyl maleimide (NEM)

Antibody Isotype




Tissue of Origin


Culture Conditions

Cell Type


Subculture Routine

Seed at 5 x 104 cells/ml. Subculture at 5 x 105 cells/ml (usually after 3-4 days). 5% CO2/95% air, 37°C.

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode


Additional Info


Depositor: Neil Barclay, Sir William Dunn School of Pathology, University of Oxford, OX1 3RE. Deposited by: LifeArc (MRC Technology), 7th Floor Lynton House, 7-12 Tavistock Square, London, WC1H 9LT

Country of Origin

United Kingdom


None specified by depositor

GMO Status

Not Applicable

Hazard Group (ACDP)




Holbrook LM, Kwong LS, Metcalfe CL, Fenouillet E, Jones IM, Barclay AN. OX133, a monoclonal antibody recognizing protein-bound N-ethylmaleimide for the identification of reduced disulfide bonds in proteins. MABS (2016);8:672-7. DOI: 10.1080/19420862.2016.1152443

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: 

OX-133 (ECACC 15090201)

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.


ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.