Cell Line Name
Cell Line Description
The hybridoma cell line OX-133 secretes mouse monoclonal IgG antibodies reactive with N-ethyl maleimide (NEM) modified proteins
Also known as
Unique to ECACC
Recombinant protein reduced and then alkylated with N-ethyl maleimide (NEM)
Tissue of Origin
Seed at 5 x 104 cells/ml. Subculture at 5 x 105 cells/ml (usually after 3-4 days). 5% CO2/95% air, 37°C.
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Country of Origin
Hazard Group (ACDP)
Holbrook LM, Kwong LS, Metcalfe CL, Fenouillet E, Jones IM, Barclay AN. OX133, a monoclonal antibody recognizing protein-bound N-ethylmaleimide for the identification of reduced disulfide bonds in proteins. MABS (2016);8:672-7. DOI: 10.1080/19420862.2016.1152443
If use of this culture results in a scientific publication, it should be cited in the publication as:
OX-133 (ECACC 15090201)
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.