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Catalogue No.


Cell Line Name


Cell Line Description

The hybridoma OX-47 secretes mouse monoclonal IgG1 antibody reactive with rat CD147 membrane protein

General Info

Fusion Species

Mouse x Mouse Hybridoma

Target (Reactivity)

Rat CD147

Also known as


Unique to ECACC


Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.





Activated rat lymphocytes

Immunological Donor

BALBc mouse spleen

Antibody Isotype




Tissue of Origin


Culture Conditions

Cell Type


Subculture Routine

Seed at 5 x 10⁴ cells/ml. Subculture When cells reach 5 x 10⁵ cells/ml (usually after 3-4 days); 5% CO₂; 37°C

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode


Additional Info


Neil Barclay, Sir William Dunn School of Pathology, University of Oxford.

Country of Origin

United Kingdom


This antibody binds to activated rat T-lymphocytes. It labels all T and B cell strongly and thoracic duct lymphocytes and thymocytes weakly. The antibody labels vascular endothelium, kidney tubules and brain capillaries but not large vessel endothelia.


Detection of CD147

Hazard Group (ACDP)




Paterson et al, 1987 Mol Immunol 24:1281 Fossum S, Mallett S, Barclay AN. Eur J Immunol. 1991 Mar; 21(3):671-9. PMID:2009910

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as OX-47 (ECACC 15090220)

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.