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UK Health Security Agency

DLKP-M

DLKP-M

Catalogue No.

20082603

Cell Line Name

DLKP-M

Cell Line Description

DLKP-M (mesenchymal-like) is a morphologically distinct subpopulation of a poorly differentiated lung carcinoma DLKP (ECACC Acc. 20082601). DLKP was established from a lymph node biopsy of a 52y old male. On prolonged subculture, DLKP-SQ (ECACC Acc. 20082604) and DLKP-M can each interconvert with DLKP-I (ECACC Acc. 20082602), but SQ and M do not interconvert.

Clonal subpopulation of DLKP used in drug resistance studies, it makes up approximately 5% of the cell population of DLKP. Parental DLKP displays chromosomal deletions and rearrangements (translocations and isochromosomes). Most similar to parent; 60% hyperdiploid.

In isolation, these cells are of intermediate size, with irregular elongated shapes and possess short neurite-like processes. They do not form colonies in monolayer culture, but instead grow in a scattered manner, with some cells extending across neighbouring cells. In the mixed parental population, these cells have a more regular flattened appearance, but again, they do not form colonies.

Cells have a doubling time of about 20 hrs.

It is tumerogenic in SCID-Mice strain CB17/lcr-Prkdc scid.

Characteristics

Tissue of Origin

Poorly differentiated lung carcinoma, established from a lymph node biopsy.

Morphology

Epithelial

DNA profile (STR Profile)

Amelogenin: X, Y
CSF1PO: 11, 12
D3S1358: 17, 17
D5S818: 11, 11
D7S820: 8, 8
D8S1179: 13, 14
D13S317: 12, 12
D16S539: 10, 12
D18S51: 13, 13
FGA: 20, 20
Penta D: 11, 11
Penta E: 7, 15
TH01: 9.3, 9.3
TPOX: 8, 11
vWA: 17, 17

Karyotype

Parental DLKP displays chromosomal deletions and rearrangements (translocations and isochromosomes). Most similar to parent; 60% hyperdiploid. Chromosome numbers were determined by microscopic analysis. At least 50 metaphase spreads from each cell line were counted.

Applications

Clonal subpopulation of DLKP used in drug resistance studies.

Disease

Squamous Cell Carcinoma

Culture Conditions

Cell Type

Poorly differentiated squamous

Subculture Routine

Upon resuscitation count cells and spin to remove DMSO.

Seed at the higher end of the seeding density to establish culture. Feed with fresh growth medium the following day. Doubling time about 20 hrs.

Seed cells between 2-4x10e4 cells/cm2 and feed twice a week.

Incubate at 8% CO2 at 37°C (as medium is a mixture of DMEM/F-12 Ham's). Trypsinise using Trypsin/EDTA.

Culture Medium

DMEM:Hams F12 + 5% Foetal Bovine Serum (FBS) + 2mM L-Glutamine

Freeze in 5% DMSO; 45% FBS; 50% growth medium.

Growth Mode

Adherent

Additional Info

Depositor

Depositor: Martin Clynes: National Institute for Cellular Biotechnology, Dublin City University. Originator: Shirley McBride (1997): National Institute for Cellular Biotechnology (formerly National Cell and Tissue Culture Centre), Dublin City University.

Country of Origin

Ireland

GMO Status

Not Applicable

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

S. McBride, P. Meleady, A. Baird, D. Dinsdale, M. Clynes, Human lung carcinoma cell line DLKP contains 3 distinct subpopulations with different growth and attachment properties, Tumour Biol.19 (1998) 88–103. PMID: 9486560

Bibliography

Keenan J, Joyce H, Aherne S, O’Dea S, Doolan P, Lynch V, Clynes M (2012). Exp Cell Res 318(5):593–602.

 

O'Sullivan D, Henry M, Joyce H, et al. Tumour Biol. 2014;35(7):6983–6997.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: DLKP-M (ECACC 20082603).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

 

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.