Catalogue No.
20082604
DLKP-SQ
DLKP-SQ
Cell Line Name
DLKP-SQ
Cell Line Description
Squamous-like subpopulation of DLKP (ECACC Acc. 20082601); a poorly differentiated lung carcinoma. The original line was established from a lymph node biopsy of a 52y old male who smoked approximately 40 cigarettes a day for most of his adult life, and was diagnosed with poorly differentiated squamous cell lung carcinoma.
DLKP-SQ was cloned once, where as the other subpopulations DLKP-I (ECACC Acc. 20022602) and DLKP-M (ECACC Acc. 20082603) were cloned and then re-cloned. The DLKP-SQ morphology appears to predominate in the parental DLKP population (~70%). These cells are relatively large squamous-like cells which form colonies with distinct cell boundaries in monolayer culture.
General Info
Species
Human
Unique to ECACC
No
Also Known As
DLKP-SQuamous clone
Other Collection No.
CancerTools 157659
Links
Nagoya Status
Not Applicable
Release Conditions
Characteristics
Products
STR profiling confirmed that DLKP-SQ was derived from DLKP. Clonal subpopulation of DLKP used in drug resistance studies, grows readily without a surface for attachment.
Tissue of Origin
Lymph node metastasis of a primary lung tumour.
Morphology
Epithelial, ‘cobble-stone’ appearance
DNA profile (STR Profile)
Amelogenin: X, Y
CSF1PO: 12, 12
D3S1358: 17, 17
D5S818: 10, 11
D7S820: 8, 11
D8S1179: 13, 14
D13S317: 12, 12
D16S539: 10, 12
D18S51: 13, 13
FGA: 20, 20
Penta D: 11, 11
Penta E: 7, 15
TH01: 9.3, 9.3
TPOX: 8, 11
vWA: 17, 17
Karyotype
Parent DLKP displays chromosomal deletions and rearrangements (translocations and isochromosomes). DLKP-SQ is more hypertetraploid than DLKP-M, and was found to be the most varied in chromosome numbers of the three clones of DLKP (SQ, I or M). 36% of the cells had modal numbers between 90 and 100, while 27% had numbers between 100 and 110.
Applications
Drug resistance studies.
Disease
Lung squamous cell carcinoma
Culture Conditions
Cell Type
Poorly differentiated squamous
Subculture Routine
Upon resuscitation count cells and seed at the higher end of the seeding density to establish culture. Feed with fresh growth medium the following day. Doubling time ~20 hrs.
Seed cells between 2-4x104 cells/cm2 and feed twice a week.
Incubate at 8% CO2 at 37°C (as medium is a mixture of DMEM/F-12 Ham's). Trypsinise using Trypsin/EDTA.
Culture Medium
DMEM:Ham's F-12 (1:1) + 5% Foetal Bovine Serum (FBS) + 2mM L-Glutamine
Freeze in 5% DMSO + 45% FBS + 50% growth medium
Growth Mode
Adherent
Additional Info
Depositor
Martin Clynes, National Institute for Cellular Biotechnology, Dublin City University. Originator: Geraldine Grant (1989): National Institute for Cellular Biotechnology (formerly National Cell and Tissue Culture Centre), Dublin City University.
Country of Origin
Ireland
GMO Status
Not Applicable
Hazard Group (ACDP)
Hazard Group (ACDP) 2
Applications
References
S. McBride, P. Meleady, A. Baird, D. Dinsdale, M. Clynes. (1998). Human lung carcinoma cell line DLKP contains 3 distinct subpopulations with different growth and attachment properties, Tumour Biol.19. pp88–103. PMID:9486560
Bibliography
Keenan J, Joyce H, Aherne S, O’Dea S, Doolan P, Lynch V, Clynes M (2012). Exp Cell Res 318(5):593–602.
O'Loughlin, C, Heenan, M, Coyle, S and Clynes, M. (2000). Altered cell cycle response of drug-resistant lung carcinoma cells to doxorubicin. Eurp J Cancer. 36: pp1149-1160.
NicAmhlaoibh R, Heenan M, Cleary I, et al. (1999) Int J Cancer. 82(3): pp368–376.
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: DLKP-SQ (ECACC 20082604).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.