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UK Health Security Agency

DLKP-A10

DLKP-A10

Catalogue No.

20082609

Cell Line Name

DLKP-A10

Cell Line Description

Highly Adriamycin-resistant variant of DLKP-A (ECACC Acc. 20082607). DLKP-A10 is a poorly differentiated quamous cell carcinoma isolated from a lymph node metastases of lung carcinoma from a 52 year old male. DLKP (ECACC Acc. 20082601) (the parent line of DLKP-A) was established from a patient that smoked approximately 40 cigarettes a day for most of his adult life.

This variant of DLKP-A was derived by exposure to increasing concentrations of Adriamycin up to 10 μg/ml. It overexpresses the MDR associated P-glycoprotein efflux pump.

Characteristics

Products

The cell line exhibits various levels of resistance to chemotherapeutic agents. Variant of DLKP-A developed by incremental exposure to increasing concentrations of Adriamycin to 10 μg/ml. Expresses PGP. IC50 for Adriamycin 13,985 ng/ml, 765-fold more resistant than parent.

Tissue of Origin

Poorly differentiated lung carcinoma, established from a lymph node biopsy.

Morphology

Epithelial

DNA profile (STR Profile)

Amelogenin: X, Y
CSF1PO: 12, 12
D3S1358: 17, 17
D5S818: 10, 11
D7S820: 8, 11
D8S1179: 13, 14
D13S317: 12, 12
D16S539: 10, 12
D18S51: 13, 13
FGA: 20, 20
Penta D: 11, 11
Penta E: 7, 15
TH01: 9.3, 9.3
TPOX: 8, 11
vWA: 17, 17

Karyotype

Parental DLKP displays chromosomal deletions and rearrangements (translocations and isochromosomes)

Applications

Drug resistance studies

Disease

Lung squamous cell carcinoma

Culture Conditions

Cell Type

Poorly differentiated squamous

Subculture Routine

Upon resuscitation count cells and seed at the higher end of the seeding density to establish culture. Feed with fresh growth medium the following day.

Seed cells between 1-4 x 104 cells/cm2.

Incubate at 8% CO2 at 37°C (as medium is a mixture of DMEM/Ham's F12). Trypsinise using Trypsin/EDTA.

 

Depositor recommends exposing to 10 μg/ml Adriamycin every fourth passage to maintain resistance, ideally removing before freezing. Upon passaging it is recommended that cells are left for 24 hrs before the addition of Adriamycin to allow cells to plate down before exposure.

Culture Medium

DMEM:Ham's F-12 (1:1) + 5% Foetal Bovine serum (FBS) + 2mM L-Glutamine

Freeze in 5% DMSO + 45% FBS + 50% growth medium.

Growth Mode

Adherent

Additional Info

Depositor

Martin Clynes, National Institute for Cellular Biotechnology, Dublin City University. Originator: Geraldine Grant (1989): National Institute for Cellular Biotechnology (formerly National Cell and Tissue Culture Centre), Dublin City University.

Country of Origin

Ireland

GMO Status

Not Applicable

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Cleary I, Doherty G, Moran E, Clynes M. (1997). The multidrug-resistant human lung tumour cell line, DLKP-A10, expresses novel drug accumulation and sequestration systems. Biochem Pharmacol. 53(10): pp1493–1502.
doi:10.1016/s0006-2952(97)00003-8 PMID:9260877

Bibliography

Keenan J, Murphy L, Henry M, Meleady P, Clynes M. (2009). Proteomic analysis of multidrug-resistance mechanisms in adriamycin-resistant variants of DLKP, a squamous lung cancer cell line. Proteomics 9: pp1556–1566. PMID:19242932

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: DLKP-A10 (ECACC 20082609).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

 

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.