Shmac 4


Shmac 4
Cell Line Name
Cell Line Description
Shmac 4 is an immortalised human prostate cell line derived from a biopsy of a well-differentiated early stage prostate cancer (Gleason grade (1+2)). Shmac 4 was immortalised using human papillomavirus type E6 gene from PLXSN16E6 retroviral stock (Denise Galloway, Fred Hutchinson Cancer Centre Seattle)
General Info
Species
Human
Links
Cellosaurus: CVCL_2722
Release Conditions
Characteristics
Receptors
Products
Tissue of Origin
DNA profile (STR Profile)
Amelogenin: X,Y
CSF1PO: 12
D5S818: 12,13
D7S820: 7,10
D13S317: 11
D16S539: 11,14
TH01: 7,9
TPOX: 8
vWA: 17,19
Karyotype
Disease
Culture Conditions
Cell Type
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.
Culture Medium
Keratinocyte Growth Medium 2 + Supplements (Sigma C-20011) + 2mM L-Glutamine + 2% Foetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability.
Growth Mode
Additional Info
Depositor
Country of Origin
GMO Status
Hazard Group (ACDP)
Applications
References
Lang SH, et.al; (2006) Differentiation of prostate epithelial cell cultures by matrigel/stromal cell glandular reconstruction. In Vitro Cell Dev Biol Anim.42:273-80 PMID: 17163777
Documents
- 10112302_Shmac 4_Growth Profile
- Sigma S0196_Data sheet_Shmac 4
- Sigma S9945_Data sheet_Shmac 4
- 10112302_Shmac_4_Images
- P4E6 Cell Line Release Authorisation for Research Use in Commercial Organisations
- Passage numbers explained
- MSDS for nucleic acids
- MSDS For Frozen Cell Culture
- Important Technical Information for Successful Culture of Your Cells
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: Shmac 4 (ECACC 10112302).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.