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HCA-7 Colony 30

HCA-7 Colony 30

Catalogue No.


Cell Line Name

HCA-7 Colony 30

Cell Line Description

Subpopulation isolated from the HCA-7 cell line

General Info



Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.



Not specified


Not specified

Tissue of Origin


DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,15
D5S818: 10,12
D7S820: 11,12
D13S317: 9
D16S539: 10,11
TH01: 6,7
TPOX: 8,10
vWA: 14,16


Near diploid


Biology of human colorectal carcinoma



Culture Conditions

Cell Type


Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 weekly using 0.0125% trypsin/EDTA; 7.5% CO₂; 37°C. Suggested seeding density 2-4x10,000 cells/cm².

Culture Medium

DMEM (High glucose Sigma D6546) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode


Additional Info


Dr S Kirkland, Department of Histopathology, Imperial College London, Hammersmith Campus, DuCane Road London W12 0NN

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2



Kirkland & Bailey 1986 Establishment and characterisation of six human colorectal adenocarcinoma cell lines. Br J Cancer 53: 779-785. PMID: 3718830


Marsh et al., 1993 Isolation and Characterization of Multiple Cell Type from a Single Human Colonic Carcinoma: Tumourigenicity of These Cell Types in a Xenograft System. Journal of Pathology 170: 441-450. PMID: 8410493 Kirkland et al., 1995 Organisation and gel contraction by human colonic carcinoma (HCA-7) sublines grown in 3-dimensional collagen gel. Int. J. Cancer 60:877-882. PMID: 7896461

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: HCA-7 Colony 30 (ECACC 09071516).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.