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Catalogue No.


Cell Line Name


Cell Line Description

Established from a sigmoid colon adenocarcinoma of a 53 year-old female patient. Imperial College Innovations has stated that this cell line is not to be supplied to commercial organisations.

General Info



Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.




Tissue of Origin


DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,12
D5S818: 11,12
D7S820: 10,14
D13S317: 10,11
D16S539: 11,13
TH01: 6,9.3
TPOX: 8,9
vWA: 17


Not specified



Culture Conditions

Subculture Routine

Cells grow as small dense islands and will not form a monolayer. Recommended that the cell line should be subcultured approximately every 8-11 days.

Seed at approximately 6 x 10,000 cells/cm² when resuscitating from frozen. During subculture, seed cells at 2-4x10,000 cells/cm² and use 0.05% trypsin or trypsin/EDTA as a dissociation reagent. Detached cells will often remain in clumps which may be further disaggregated by repeated pipetting of the cells.

Recommended growth conditions 7.5% CO2; 37°C.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode


Additional Info


Dr S Kirkland, Department of Histopathology, Imperial College London, Hammersmith Campus, DuCane Road, London, W12 0NN

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2



S. C. Kirkland and I. G. Bailey. (1986) Establishment and characterisation of six human colorectal adenocarcinoma cell lines. Br J Cancer, 53, 6, 779-85.


P53 DeletionY. Liu and W.F. Bodmer (2006)Analysis of P53 mutations and their expression in 56 colorectal cancer cell lines.PNAS 103, 976-981.Beta-catenin MutationM. Ilyas, I.P.M. Tomlinson, A. Rowan, M. Pignatelli and W.F. Bodmer (1997)Beta-catenin mutations in cell lines established from human colorectal cancers.PNAS 94, 10330-10334.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: HCA-46 (ECACC 07031601).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.