skip to main content

A375-C6

A375-C6

Catalogue No.

97011321

Cell Line Name

A375-C6

Cell Line Description

The cell line A375-C6 has been shown to be sensitive to the IL-1 mediated anti-proliferative effect. It has been derived from the parental line A375 (ECACC catalogue no. 88113005) by limiting dilution together with its twin clone A375-C5 (ECACC catalogue no. 97011320) which is IL-1 unresponsive. A375-C6 could be useful for a growth inhibition assay for IL-1. The cells have been eradicated from mycoplasma at ECACC.

Characteristics

Receptors

IL-1 receptor

Tissue of Origin

Skin

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 12
D5S818: 12
D7S820: 9
D13S317: 11
D16S539: 9
TH01: 8
TPOX: 8,10
vWA: 16,17

Karyotype

Not specified

Applications

IL-1 growth inhibition assay, IL-1 receptor analysis

Disease

Melanoma

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 1-3 x 10,000 cells/cm²using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr K Onozaki, Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan

Country of Origin

Japan

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Onozaki K, Matsushima K, Aggarwal BB, Oppenheim JJ 1985 Human interleukin 1 is a cytocidal factor for several tumor cell lines. J Immunol. 135(6):3962-8 PMID: 2415593.

Bibliography

Endo Y, Matsushima K, Onozaki K, Oppenheim JJ 1988 Role of ornithine decarboxylase in the regulation of cell growth by IL-1 and tumor necrosis factor. J Immunol. 141(7):2342-8 PMID: 2971724. Yang D, Hayashi H, Hiyama Y, Takii T, Onozaki K 1995 Transfection of human melanoma cells with type I interleukin-1 (IL-1) receptor cDNA rendered them IL-1-responsive and revealed the importance of ODC activity down-regulation in IL-1-induced growth inhibition. J Biochem. 118(4):802-9 PMID: 8576096.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: A375-C6 (ECACC 97011321).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.