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F4N (MEL)

F4N (MEL)

Catalogue No.

96121718

Cell Line Name

F4N (MEL)

Cell Line Description

The mouse erythroleukemia cell line F4N(MEL) was established in 1977. They lost the ability for erythroid differentiation by induction with DMSO or other reagents. Eight percent of the total number of cells in culture undergo spontaneous apoptosis.

Characteristics

Tissue of Origin

Blood

Morphology

Round

Karyotype

Aneuploid

Applications

Apoptosis and DNA fragmentation studies

Disease

Erythroleukaemia

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells / ml; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Dr Audza Kalvelyte, Institute of Biohemistry, Vilnius, Lithuania

Country of Origin

Lithuania

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

DNA Cell Biol 1996; 15:287

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: F4N (MEL) (ECACC 96121718).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.