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BHK/AC9

BHK/AC9

Catalogue No.

85040103

Cell Line Name

BHK/AC9

Cell Line Description

BHK/AC9 is derived from the parent line BHK-21 (Clone-13) (ECACC catalogue no. 85011433). The cells have been adapted to grow in suspension and have the same virus susceptibility as the parent line.

Characteristics

Tissue of Origin

Kidney

Karyotype

2n = 44, pseudodiploid

Applications

Virus replication studies

Disease

None Stated

Culture Conditions

Subculture Routine

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37°C.

Culture Medium

GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 5-10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Dr P Whiteside, Deparment of Microbiology, University of Surrey

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Arch Virol 1980;63

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: BHK/AC9 (ECACC 85040103).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.