GPNT
GPNT
Cell Line Name
Cell Line Description
GPNT was derived from the immortalised rat brain endothelial cell line GP8 by lipofectin-mediated transfection with a vector containing the puromycin resistance gene. GPNT exhibits a non-transformed phenotype.
General Info
Species
Rat
Links
Cellosaurus: CVCL_T962
Release Conditions
Characteristics
Receptors
Products
Tissue of Origin
Morphology
Applications
GPNT is a cell model for cerebral endothelium used to study the blood-brain barrier.
Disease
Culture Conditions
Cell Type
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 10hrs.
Culture Medium
Hams F10 + 10% Heat-inactivated FCS + 2mM Glutamine + 2ng/ml basic fibroblast growth factor + 80µg/ml heparin. All plasticware is pre-coated for several hours with collagen (Type I from calf skin) at 10µg/cm² from a 0.01% solution in acetic acid. After removal the coated plasticware is exposed to ammonia vapour for 5 min. Plasticware must be rinsed thoroughly with PBS prior to use.
Growth Mode
Additional Info
Depositor
Country of Origin
GMO Status
Hazard Group (ACDP)
Applications
References
Greenwood et al., (1996) SV40 large T immortalised cell lines of the rat blood-brain and blood-retinal barriers retain their phenotypic and immunological characteristics. Journal of Neuroimmunology 71: 51-63. PMID: 8982103.
Bibliography
Regina et al., (1999) Dexamethasone regulation of P-glycoprotein activity in an immortalized rat brain endothelial cell line, GPNT. Journal of Neurochemistry 73: 1954-1963. PMID:10537053.
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: GPNT (ECACC 09061601).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.