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UK Health Security Agency

tsDC

tsDC

Catalogue No.

01081609

Cell Line Name

tsDC

Cell Line Description

This is a conditionally immortalised dendritic cell line which was established from bone marrow of CBA (H-2k) mice transgenic for a thermo labile mutant of the SV40 large T antigen under the control of the Class I Kb promoter. At 33-37°C it divides in the absence of GM-CSF. It shares a number of cell surface markers with bone marrow macrophages, but unlike macrophages is constitutively MHC Class II+. Transfer to 39°C, arrests growth and results in up-regulation of surface markers such as B7.1, CD40 and intercellular adhesion molecule-1. Mycoplasma eradicated prior to deposit at ECACC.

General Info

Species

Mouse

Release Conditions

Restricted – all customers must complete the specific depositor MTA.

Characteristics

Tissue of Origin

Bone marrow

Karyotype

Not specified

Applications

The tsDC line should facilitate further analysis of the signals and molecules required for terminal differentiation

Disease

No associated disease stated

Culture Conditions

Subculture Routine

Passage cells approximately every 1- 2 weeks. Culture at 33°C; 5-10% CO₂. Cells have heterogenous morphology, but the line has been cloned. Most cells adhere but some also grow in suspension. Split sub-confluent cultures (70-80%) . Collect any suspension cells, then remove the attached cells via a PBS wash followed by short incubation in 20mM EDTA. Once the cells have detached add culture medium in excess and centrifuge at 150g for 5 minutes.Seed new flasks at 1-3 x10,000 cells/cm². If resuscitating from a frozen ampoule seed at approximately 4 x10,000 cells/cm².

Culture Medium

Iscove's MEM + 5% Foetal Bovine Serum (FBS) + 2 mM Glutamine + 0.05 mM 2Mercaptoethanol (2ME).

Growth Mode

Semi-adherent

Additional Info

Depositor

Dr Brigitta Stockinger, Division of Molecular Immunology, National Institute for Medical Research, The Ridgeway, Mill Hill, London

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Volkmann A, Neefjes J, Stockinger B (1996) A conditionally immortalized dendritic cell line which differentiates in contact with T cells or T cell-derived cytokines. Eur J Immunol. 26(11):2565-72. PMID: 8921940

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: tsDC (ECACC 01081609).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.