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BF-2

BF-2

Catalogue No.

00021713

Cell Line Name

BF-2

Cell Line Description

The cell line BF-2 has been derived from a trypsinised suspension of pooled caudal portions of the trunk of 1 year old fingerlings (Bluegill fry, Lepomis macrochirus). Cells have been reported to be susceptible to a variety of fish viruses including viral haemorrhagic septicaemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV), spring viraemia of carp virus (SVCV) and pike fry rhabdovirus (PFRV).

Characteristics

Tissue of Origin

Caudal trunk

Karyotype

Not specified

Applications

Fish virus studies

Disease

None Stated

Culture Conditions

Cell Type

Fibroblast-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:6 using 0.05% trypsin or trypsin/EDTA; cells replicate at 15 - 33°C, optimal temperature is 23 - 26°C; monolayer is stable for at least 10 days in maintenance medium (at 15 or 16°C for about 21 days). Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 media changes before shipping.

Culture Medium

MEM (HBSS) or MEM (EBSS) + 2mM Glutamine + 850 mg/L NaHCO₃ + 1% NEAA + 120 mg/l NaPy + 10% FBS; a mixture of MEM (HBSS) and MEM (EBSS) with 1-2% FBS is best for stability of monolayer and maintenance medium

Growth Mode

Adherent

Additional Info

Depositor

Dr. R. Riebe, Collection of Cell Lines in Veterinary Medicine (CCLV) Federal Research Centre for Virus Diseases of Animals, 17498 Island Riems, Germany

Country of Origin

Germany

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Science 1966; 151:1004, J Virol 1968; 2:393, J Infect Dis 1968; 11:253, In Vitro Cell Dev. Biol 1992; 28A:385

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: BF-2 (ECACC 00021713).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.