Extended Bibliography: |
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Ref #: |
16329 |
Author(s): |
Ramos,C.P.;Foster,G.;Collins,M.D. |
Journal: |
Int J Syst Bacteriol |
Title: |
Phylogenetic analysis of the genus Actinomyces based on 16S rRNA gene sequences: description of Arcanobacterium phocae sp. nov., Arcanobacterium bernardiae comb. nov., and Arcanobacterium pyogenes comb. nov |
Volume: |
47 |
Page(s): |
46-53 |
Year: |
1997 |
Keyword(s): |
GENBANK/X79225
GENBANK/X80411
GENBANK/X80412
GENBANK/X80413
GENBANK/X80414
GENBANK/X81061
GENBANK/X81062
GENBANK/X81063
GENBANK/X82448
GENBANK/X82449
GENBANK/X82450
GENBANK/X82451
GENBANK/X82452
GENBANK/X82453
GENBANK/X97049
Actinomycetaceae/*classification/*genetics
Bacteriological Techniques
Culture Media/metabolism
DNA, Bacterial/*analysis
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S/*genetics
Sequence Homology, Nucleic Acid
|
Remarks: |
A systematic phylogenetic analysis of the genus Actinomyces was performed. The 16S rRNA gene sequences of 13 Actinomyces species, an unnamed Actinomyces strain (ATCC 49338), and an Actinomyces-like isolate from sea mammals were determined. Comparative sequence analysis with closely related taxa revealed phylogenetic diversity and internal structure within the genus Actinomyces. In addition, some members of other genera (viz., the genera Arcanobacterium, Mobiluncus, and Rothia) were shown to be phylogenetically intermixed with the Actinomyces species. It was evident from both distance and tree topology considerations that the genus Actinomyces is in urgent need of taxonomic revision and requires subdivision into several genera. Based on the results of the present study it is proposed that Actinomyces bernardiae and Actinomyces pyogenes be assigned to the genus Arcanobacterium as Arcanobacterium bernardiae comb. nov. and Arcanobacterium pyogenes comb. nov., respectively. In addition, a new species, Arcanobacterium phocae, is proposed for Actinomyces-like bacteria isolated from seals. |
URL: |
8995800 |
|
Ref #: |
16452 |
Author(s): |
Jauh-Hsun,C.;Vinh,T.;Davies,J.K.;Figdor,D. |
Journal: |
Oral Microbiol Immunol |
Title: |
Molecular approaches to the differentiation of Actinomyces species |
Volume: |
14 |
Page(s): |
250-6 |
Year: |
1999 |
Keyword(s): |
Actinomyces/*classification/*genetics
Bacterial Typing Techniques
DNA Probes
DNA, Bacterial/analysis
Oligonucleotide Probes
RNA, Ribosomal, 16S/analysis
Sequence Analysis, DNA
Sequence Analysis, RNA
Species Specificity
|
Remarks: |
The definition of the genus Actinomyces relies heavily on traditional methods of taxonomy. This study sought to develop molecular tools for the identification of strains of Actinomyces israelii and Actinomyces gerencseriae. Oligonucleotide probes were designed and one of these successfully differentiated. A. gerencseriae from ten strains of A. israelii and three other Actinomyces species by DNA:DNA hybridization. However, probes based on known 16S rRNA sequences failed to hybridize to all the strains previously identified as A. israelii. Using the PCR technique, a region encoding a portion of the 16S rRNA was amplified from genomic DNA. The results showed that A. israelii can be divided into three different groups based on comparison of the amplified DNA sequences. This information should allow the development of probes that are specific for these newly identified groups of strains within the species A. israelii. |
URL: |
10551170 |
|
Ref #: |
12011 |
Author(s): |
Ramos,C.P.;Foster,G.;Collins,M.D. |
Journal: |
Int J Syst Bacteriol |
Title: |
Phylogenetic analysis of the genus Actinomyces based on 16S rRNA gene sequences: description of Arcanobacterium phocae sp. nov., Arcanobacterium bernardiae comb. nov., and Arcanobacterium pyogenes comb. nov |
Volume: |
47 |
Page(s): |
46-53 |
Year: |
1997 |
Keyword(s): |
GENBANK/X79225
GENBANK/X80411
GENBANK/X80412
GENBANK/X80413
GENBANK/X80414
GENBANK/X81061
GENBANK/X81062
GENBANK/X81063
GENBANK/X82448
GENBANK/X82449
GENBANK/X82450
GENBANK/X82451
GENBANK/X82452
GENBANK/X82453
GENBANK/X97049
Actinomycetaceae/*classification/*genetics
Bacteriological Techniques
Culture Media/metabolism
DNA, Bacterial/*analysis
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S/*genetics
Sequence Homology, Nucleic Acid
Support, Non-U.S. Gov't
|
Remarks: |
A systematic phylogenetic analysis of the genus Actinomyces was performed. The 16S rRNA gene sequences of 13 Actinomyces species, an unnamed Actinomyces strain (ATCC 49338), and an Actinomyces-like isolate from sea mammals were determined. Comparative sequence analysis with closely related taxa revealed phylogenetic diversity and internal structure within the genus Actinomyces. In addition, some members of other genera (viz., the genera Arcanobacterium, Mobiluncus, and Rothia) were shown to be phylogenetically intermixed with the Actinomyces species. It was evident from both distance and tree topology considerations that the genus Actinomyces is in urgent need of taxonomic revision and requires subdivision into several genera. Based on the results of the present study it is proposed that Actinomyces bernardiae and Actinomyces pyogenes be assigned to the genus Arcanobacterium as Arcanobacterium bernardiae comb. nov. and Arcanobacterium pyogenes comb. nov., respectively. In addition, a new species, Arcanobacterium phocae, is proposed for Actinomyces-like bacteria isolated from seals. |
URL: |
97148975 |
|
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