skip to main content



Catalogue No.


Cell Line Name


Cell Line Description

Although originally described as a colon adenocarcinoma line established from a 78 year old female, DNA fingerprinting has shown this line to be a derivative of HT-29 (Chen TR, Drabkowski D, Hay RJ, Macy M, Peterson W Jr. 1987 WiDr is a derivative of another colon adenocarcinoma cell line, HT-29. Cancer Genet Cytogenet. 27(1):125-34. PMID: 3472642). HT29 was isolated from a primary colon adenocarcinoma in a 44 year old Caucasian female. The cells produce carcinoembryonic antigen and are tumorigenic in xenogeneic animals. They have been used in studies of anti-tumour agents.



Carcinoembryonic antigen (CEA)

Tissue of Origin


DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,12
D5S818: 11,12
D7S820: 10
D13S317: 11,12
D16S539: 11,12
TH01: 6,9
TPOX: 8,9
vWA: 17,19


2n = 46, hypertriploid


Carcinogenesis and tumourigenicity studies and anti-tumour testing



Culture Conditions

Cell Type


Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode


Additional Info


Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2



In Vitro 1979;15:401; Cancer Res 1974;34:105; Cancer Res 1978;38:3745, Science 1985; 230:943-945, Cytogenet. 1987; 27:125-134, Cancer Res 1993; 53:5697-5706, Proc. Natl. Acad. Sci USA 1990; 87:7555-7559, Science 1978; 199:980-983


Cancer Res 1998; 58:109-113, Cancer Res. 1997; 57:5528-5533

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: WiDr (ECACC 85111501).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.