Cell Line Name
Cell Line Description
T35J-5/09 is clonally-derived from a CHO-K1 cell line which has been transfected with a human free fatty acid receptor 3 G Protein Coupled Receptor (GPCR) cassette to allow expression of the human receptor 3 GPCR T35J-5/09 is an example of a cell line transfected using InSCREENeX SCREENflexTM recombinase mediated cassette exchange (RMCE) methodology (see www.inscreenex.com).
Also Known As
Tissue of Origin
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
Ham's F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Country of Origin
Hazard Group (ACDP)
Schucht R, Coroadinha AS, Zanta-Boussif MA, Verhoeyen E, Carrondo MJ, Hauser H, Wirth D. (2006) A new generation of retroviral producer cells: predictable and stable virus production by Flp-mediated site-specific integration of retroviral vectors. Mol Ther. 14(2):285-92 PMID: 16697259.
Nehlsen K, Schucht R, da Gama-Norton L, KrÃ¶mer W, Baer A, Cayli A, Hauser H, Wirth D. (2009) Recombinant protein expression by targeting pre-selected chromosomal loci. BMC Biotechnol. 9:100 PMID: 20003421. Schucht R, Wirth D, May T. (2010) Precise regulation of transgene expression level and control of cell physiology. Cell Biol Toxicol. 26(1):29-42 PMID: 19763851.
If use of this culture results in a scientific publication, it should be cited in the publication as: T35J-5/09 (ECACC 10031602).
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.