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PORTHOS

PORTHOS

Catalogue No.

93122323

Cell Line Name

PORTHOS

Cell Line Description

Derived from porcine thyroid. The cells have been adapted to grow in low serum (0.1%) media.

Characteristics

Receptors

Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone

Products

Thyroglobulin, plasminogen activator (u-PA)

Tissue of Origin

Thyroid gland

Karyotype

Not specified

Applications

Regulation of thyroid cell metabolism

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 seeding at 2 x 10 4 cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO₂; 37°C.

Culture Medium

Click:RPMI (1:1) + 10µg/ml Insulin + 10mM Hydrocortisone + 5µg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 10mU/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr G Fayet/Dr S Hovsepian, Laboratoire de Biochimie Medicale, Faculte de Medecine, Marseille, F-13385

Country of Origin

France

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Fayet G and Hovsepian S (1985) In: Progress in Endocrine Research and Therapy, vol 2, Thyroglobin - The Prothyroid Hormone (eds) Burrow GN and Eggo M. Raven Press, NY. pp211-224.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: PORTHOS (ECACC 93122323).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.