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Neuro 2a

Neuro 2a

Catalogue No.


Cell Line Name

Neuro 2a

Cell Line Description

Derived from a spontaneous tumour in an albino strain A mouse. Cells produce microtubular protein which is believed to play a role in the contractile system giving axoplasmic flow in nerve cells.



Microtubular protein

Tissue of Origin





2n = 40


Tumourigenicity studies, microtubular protein synthesis, vinblastine precipitation, GTP binding kinetics



Culture Conditions

Cell Type


Subculture Routine

Split sub-confluent cultures (70-80%). The recommended seeding density for these cells is 2-4x104 cells/cm2. After resuscitation, these cells can take 3-4 days before a monolayer forms.


Recommended growth conditions 5% CO2; 37°C.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode


Additional Info


Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2



J Cell Biol 1969;43:69A; Proc Natl Acad Sci, USA 1970;65:129


Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: Neuro 2a (ECACC 89121404).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

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