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NB4 1A3

NB4 1A3

Catalogue No.


Cell Line Name

NB4 1A3

Cell Line Description

Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.



Acetylcholinesterase, choline acetylase, tyrosine hydroxylase

Tissue of Origin





Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus



Culture Conditions

Cell Type


Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.05% trypsin; 5% CO₂; 37°C.

Culture Medium

Ham's F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Growth Mode


Additional Info


Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2



Proc Natl Acad Sci, USA 1962;48:1184


Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: NB4 1A3 (ECACC 89121405).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.