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MS-1

MS-1

Catalogue No.

09111802

Cell Line Name

MS-1

Cell Line Description

MS-1 cells contain a Merkel cell polyomavirus integrated into the genome. See also MKL-1 (ECACC catalogue number 09111801).

Characteristics

Receptors

Not specified

Products

Cell line is CM2B4/MCV T, AE1/AE2, CK20, EMA, CAM5.2, chromogranin & NSE. Cell line is negative for for CK7, synaptophysin, LCA TTF-1, vimentin and CD99 ,

Tissue of Origin

Skin

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 10
D5S818: 11
D7S820: 8,9
D13S317: 11,14
D16S539: 9
TH01: 7,9.3
TPOX: 8
vWA: 17

Karyotype

Not specified

Applications

Pathobiology of neuroendocrine skin carcinomas (NSCs) and function of cutaneous Merkel cells. The role of polyomavirus in the development of Merkel cell carcinomas (submitted for publication)

Disease

Mesothelioma

Culture Conditions

Cell Type

Carcinoma

Subculture Routine

Seed at 3 x 10⁵ cells/ml in 5% CO₂; 37°C Saturation density is approximately 10⁶ cells/ml.

Culture Medium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Dr Patrick Moore, University of Pittsburgh Cancer Institute, 5117 Centre Avenue Suite 1.8 Pittsburgh PA 15213

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Feng et al., (2008) Clonal integration of a polyomavirus in human Merkel cell carcinoma. Science 319: 1096-1100. PMID: 18202256.

Bibliography

Houben R, Shuda M, Weinkam R, Schrama D, Feng H, Chang Y, Moore PS, Becker JC.(2010) Merkel cell polyomavirus-infected Merkel cell carcinoma cells require expression of viral T antigens.PMID: 20444890.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: MS-1 (ECACC 09111802).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.