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MKL-1

MKL-1

Catalogue No.

09111801

Cell Line Name

MKL-1

Cell Line Description

MKL-1 was established from a nodal metastasis in a 26-year-old Caucasian male patient. MKL-1 cells contain a Merkel cell polyomavirus integrated into the genome. MKL-1 cells cause tumour formation at 4-6 weeks at subcutaneous sites in athymic male nude mice (BALB/c nu/nu).

Characteristics

Products

MKL-1 is positive for CM2B4/MCV T AE1/AE2, CK20, EMA, CAM5.2, NSE and TTF-1. MKL-1 is negative for CK7, chromagen, synaptophysin, neurofilament, LCA, vimentin and CD99.

Tissue of Origin

Skin

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 11,12
D5S818: 11,12
D7S820: 8,11
D13S317: 8,11
D16S539: 10,12
TH01: 9,9.3
TPOX: 8
vWA: 16,18

Karyotype

Hyperdiploid

Disease

Mesothelioma

Culture Conditions

Cell Type

Carcinoma

Subculture Routine

Seed at 3 x 10⁵ cells/ml in 5% CO₂; 37°C Saturation density is approximately 10⁶ cells/ml. Population doubling time is around 120 hours.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% FBS

Growth Mode

Suspension

Additional Info

Depositor

Dr Patrick Moore, University of Pittsburgh Cancer Institute, 5117 Centre Avenue Suite 1.8 Pittsburgh PA 15213 (Originator: Dr Victor Gould & Dr James Radosevich, Department of Pathology, Rush Medical College, 1753 west Congress Parkway, Chicago, Illinois, 60612)

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Rosen et al., (1987) Establishment and characterization of a neuroendocrine skin carcinoma cell line. Laboratory Investigation 56: 302-312 PMID: 3546933.

Bibliography

Feng et al., (2008) Clonal integration of a polyomavirus in human Merkel cell carcinoma. Science 319: 1096-1100. PMID: 18202256. Shuda et al., (2008) T antigen mutations are a human tumor-specific signature for Merkel cell polyomavirus. Proc. Natl. Acad. Sci. USA 105: 16063-4 PMID: 18812503. Houben R, Shuda M, Weinkam R, Schrama D, Feng H, Chang Y, Moore PS, Becker JC.(2010) Merkel cell polyomavirus-infected Merkel cell carcinoma cells require expression of viral T antigens. J Virol. 84(14):7064-72. PMID: 20444890.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: MKL-1 (ECACC 09111801).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.