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K1

K1

Catalogue No.

92030501

Cell Line Name

K1

Cell Line Description

Derived from a primary papillary thyroid carcinoma cell line GLAG-66 (Antonini et al 1993 PMID: 8330267) (Ribeiro et al., 2008 PMID: 19087340 Schweppe et al., 2008 PMID: 18713817). Retains thyroid follicular cell differentiation e.g. thyroglobulin synthesis. Expresses wild-type p53 tumour suppresser gene.

Characteristics

Products

Wild-type p53 tumour suppressor gene

Tissue of Origin

Thyroid gland

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 11,12
D5S818: 10,11
D7S820: 11
D13S317: 11,14
D16S539: 11,12
TH01: 6,9
TPOX: 8
vWA: 17,18

Karyotype

Not specified

Applications

Tumourigenicity studies, particularly the role of p53

Disease

Carcinoma

Culture Conditions

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM:Ham's F12:MCDB 105 (2:1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr D Wynford-Thomas, Department of Pathology, University of Wales, College of Medicine

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Wyllie FS, Lemoine NR, Barton CM, Dawson T, Bond J, Wynford-Thomas D. 1993 Direct growth stimulation of normal human epithelial cells by mutant p53. Mol Carcinog: 7(2):83-8 PMID: 8457292

Bibliography

Not specified

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: K1 (ECACC 92030501).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.