skip to main content

HSG (HeLa derivative)

HSG (HeLa derivative)

Catalogue No.


Cell Line Name

HSG (HeLa derivative)

Cell Line Description

The cell line HSG was originally thought to be established from a human submandibular gland from a 54 year old Japanese male. The cell line has since been found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.



EGF, TGF-beta

Tissue of Origin



Not specified



Culture Conditions

Cell Type


Subculture Routine

Split confluent cultures 1:4 to 1:10 ie seeding at 1-3x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂, 37°C

Culture Medium

MEM +2mM Glutamine +1% Non Essential Amino Acids (NEAA) + 10% New Born Calf Serum (NBCS).

Growth Mode


Additional Info


Dr M U Iwobi/Dr A J MacGillivray, School of Biological Sciences, University of Sussex, Brighton

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2



Cancer 1981;48:745; Cancer 1984;54:2959; Cancer 1985;55:2575; Cancer Res 1985;45:6160

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: HSG (HeLa derivative) (ECACC 95031024).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.