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HF1

HF1

Catalogue No.

94101908

Cell Line Name

HF1

Cell Line Description

HF1 is a clone of hybrid cells resulting from the fusion of H5 and Fao. Expression of the majority of hepatic functions are extinguished. The cells contain a number of chromosomes corresponding to the sum of the parental numbers. HF1 variant which lacks the complete set of tissue-specific functions, appears to be a good candidate for regulatory mutants which control the orderly temporal expression of tissue specific functions during development.

Characteristics

Tissue of Origin

Liver

Karyotype

Modal no. 100

Applications

Analysis of gene expression by molecular techniques

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 9x1,000 to 2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

Coon's modified Ham's F12 (or Kaign's modified Ham's F12 + 45mg/L ascorbic acid + 18mg/L inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr M Weiss, Dept. Biologie Moleculaire, Institute Pasteur, Paris

Country of Origin

France

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Somatic Cell Genetics 1979; 5(6):697

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: HF1 (ECACC 94101908).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.