skip to main content

HeLa S3

HeLa S3

Catalogue No.


Cell Line Name

HeLa S3

Cell Line Description

Derived from the parent HeLa cell line. The cells have been adapted to grow in suspension (spinner) culture and have the same virus susceptibility as the parent line. Very useful in the clonal analysis of mammalian cell populations relating to chromosomal variation, cell nutrition and plaque forming ability. The cells should be handled under laboratory containment level 2.


Tissue of Origin


DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 9,10
D5S818: 11,12
D7S820: 8,12
D13S317: 14
D16S539: 9,10
TH01: 7
TPOX: 8,12
vWA: 16,18


Modal no. 68


Tumourigenicity and virus studies: poliovirus 1 and Ad 3. Clonal analysis of mammalian cell populations



Culture Conditions

Cell Type


Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Culture in a spinner flask. This cell line may adhere if the culture is not agitated.

Culture Medium

SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) +10% Foetal Bovine Serum (FBS)

Growth Mode


Additional Info


Mr C B Morris, PHLS CAMR, Porton Down, Salisbury

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2



J Exp Med 1956;103:273


Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: HeLa S3 (ECACC 87110901).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.