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HeB5

HeB5

Catalogue No.

11033104

Cell Line Name

HeB5

Cell Line Description

HeB5 is a derivative of Hepa-1c1c7 (ECACC Catalogue number 95090613) cell line stably transfected with a firefly luciferase reporter construct bearing Xenobiotic-Responsive Element (XRE) driven by a TATA promoter. HeB5 can be used for detection of mouse CYP1A induction by chemicals. GMO1

General Info

Species

Mouse C57L

Also Known As

Hepa-1c1c7

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Receptors

Not specified

Products

Not specified

Tissue of Origin

Liver

Karyotype

Not specified

Disease

Hepatocellular Carcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

Alpha-MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 16µg/ml Blasticidin S HCL.

Growth Mode

Adherent

Additional Info

Depositor

Dr Noriyuki Suzuki, Sumitomo chemical Co Ltd, 1-98 Kasugadenaka 3-chrome, Konohana-ku, Osaka 554-8558, Japan

Country of Origin

Japan

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

None specified by depositor

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: HeB5 (ECACC 11033104).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.