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UK Health Security Agency

FTC-238-hrTPO

FTC-238-hrTPO

Catalogue No.

25120401

Cell Line Name

FTC-238-hrTPO

Cell Line Description

The parental cell line FTC-238 (Cat.No. 94060902) was genetically modified to stably express the human thyroid peroxidase.

Tissue: Thyroid (lung metastasis). Cell line is GMO1 - TPO ORF ligated into pcDNA3 (Invitrogen). Classical transfection/lipofection and selection for G418 resistance and TPO activtiy. Used in a test system for inhibition assays.

Parental line FTC-238 came from a follicular thyroid carcinoma from a 42-year-old male.

The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Characteristics

Products

FTC-238-hrTPO has been used as source of thyroid peroxidase activity to establish test systems for respective inhibition assays.

Tissue of Origin

Thyroid (Lung metastasis)

Morphology

Polymorphic

DNA profile (STR Profile)

Amelogenin: X, X
CSF1PO: 10, 11
D3S1358: 15, 16
D5S818: 11, 12
D7S820: 9, 10
D8S1179: 10, 10
D13S317: 11, 11
D16S539: 11, 12
D18S51: 11, 12
FGA: 21, 21
Penta D: 12, 12
Penta E: 13, 13
TH01: 9.3, 9.3
TPOX: 9, 9
vWA: 18, 18

The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Applications

FTC-238-hrTPO was used as source of thyroid peroxidase activity to establish test systems for respective inhibition assays.

Disease

Carcinoma

Culture Conditions

Subculture Routine

Split sub-confluent cultures (70-80%), seeding at 0.5-3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

IMDM or DMEM:Ham's F12 (1: 1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) with the addition of 250µg/ml G418.

At ECACC this was shown to grow in both DMEM and IMDM medium.

Freeze in normal Freeze Mix (10%DMSO+90% FBS).

Growth Mode

Adherent

Additional Info

Depositor

Prof. Dr. Josef Köhrle, Institut für Experimentelle Endokrinologie, Charité Universitätsmedizin Berlin, Hessische Straße 4A; Vorderhaus, 4.OG, D-10115 Berlin, Germany

Country of Origin

Germany

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Genetic Modification

pCDNA3-plasmid with inserted ORF of human Thyroid PerOxidase gene (TPO), stably inserted into the cell line by G418 selection.

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Schmutzler, Cornelia et al. “The ultraviolet filter benzophenone 2 interferes with the thyroid hormone axis in rats and is a potent in vitro inhibitor of human recombinant thyroid peroxidase.” Endocrinology vol. 148,6 (2007): 2835-44. doi:10.1210/en.2006-1280 PMID: 17379648

Bibliography

Reinen. J. et al. (2024) Development and Standardization of an Assay to Evaluate the In Vitro Inhibition of Thyroid Peroxidase -Catalyzed Iodination Using FTC-238-hrTPO Cell Homogenates. App In Vitro Tox. 10(3): 51-64. doi: 10.1089/aivt.2024.0029

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: FTC-238-hrTPO (ECACC 25120401).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

 

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.