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UK Health Security Agency

FRTL-5

FRTL-5

Catalogue No.

91030711

Cell Line Name

FRTL-5

Cell Line Description

This thyroid cell line has been established from normal thyroid glands from 5 to 6-week-old Fischer rats. FRTL-5 is a clonal line of the continuously cultured FRTL cells derived by successive clonal isolation. The cells are characterised by maintaining highly differentiated thyroid features such as secretion of thyroglobulin and concentration of iodide. FRTL-5 cells have been used as assays measuring the stimulatory activity of human autoimmune immunoglobulins from patients with Graves disease. Tend to grow in three-dimensional structures, monolayer not formed.

Discontinued Message

The cell line does not display the non-diploid karyotype originally reported therefore its authenticity cannot be assured.

Characteristics

Products

Thyroglobulin

Tissue of Origin

Thyroid gland

Karyotype

Not specified

Applications

Study of thyroid cells, hormone dependency and secretion

Disease

None Stated

Culture Conditions

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

Coon's modified Ham's F12 medium (Biochrome Ag F0855)) + 2mM Glutamine + 10µg/ml Insulin + 10nM Hydrocortisone + 5µg/ml Transferrin + 10ng/ml gly-his-lys acetate + 10ng/ml somatostatin + 5% Foetal Bovine Serum (FBS) + 10mU/ml TSH.

Growth Mode

Adherent aggregates

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Proc Natl Acad Sci USA 1980;77:3455; Endocrinology 1983;112:71; J Endocrinol Invest 1982;5:179, Thyroid 1998;8(7):623-626

Bibliography

Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: FRTL-5 (ECACC 91030711).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.