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FM3Ats C1.T85

FM3Ats C1.T85

Catalogue No.


Cell Line Name

FM3Ats C1.T85

Cell Line Description

A temperature sensitive mutant isolated from the mouse mammary cell carcinoma cell line, FM3A (ECACC catalogue no. 87100804). Cells will not divide at 39°C (non-permissive temperature) and will grow at 33°C (permissive temperature). Electron microscope findings have shown that chromatin abnormally condenses into fragmented and compact forms in 80% of the cells at 39°C for 16 hours. This abnormal condensation does not occur at 33°C or in the FM3A cell line at either temperature.


Tissue of Origin



Not specified


Tumourigenicity studies, chromosome condensation studies



Culture Conditions

Cell Type


Subculture Routine

Maintain cultures between 1-3x100,000 cells/ml; 5% CO₂; 33°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode


Additional Info


Dr T Ohno, Riken Gene Bank, Tsukuba

Country of Origin


Hazard Group (ACDP)

Hazard Group (ACDP) 2



Exp Cell Res 1980;126:407


Not specified

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: FM3Ats C1.T85 (ECACC 87111904).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.