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Escherichia coli

Escherichia coli

Catalogue No.

NCTC 14379

Current Name

Escherichia coli

Type Strain

Reference Strain

Original Strain Reference

MC1000 + pDM1-mcr-3

Culture Conditions

Growth Conditions on Solid Media

Nutrient agar, 37°C, O₂, 24 hours

Biological and Genomic Info

Strain Data

This organism has been genetically modified and is GMO2 / pDM1 is a tetracycline selectable synthetic plasmid containing a p15a ori that is maintained at ~10 copies per cell. mcr-3 is cloned into the multiple cloning site of this plasmid, and is IPTG-inducible and driven by a Ptac promoter.pDM1 sequence: Genbank MN128719.The sequence of the mcr-3 ORF is available on request.

Additional Info

Accession Date



Despoina Mavridou (Imperial College London, Department of Life Sciences, Kensington, London, UK)

Isolated From

GMO organism derived from Escherichia coli MC1000. London, UK.


Accessioned into the NCTC < Engineered by Despoina Mavridou



M J Casadaban, S N Cohen. J Mol Biol. 1980 Apr;138(2):179-207.L Dortet et al. Rapid detection and discrimination of chromosome- and MCR-plasmid-mediated resistance to polymyxins by MALDI-TOF MS in Escherichia coli: the MALDIxin test. J Antimicrob Chemother. 2018 Dec 1;73(12):3359-3367.

Available Formats

  • Ampoule (Bacteria)

If use of this culture results in a scientific publication, it should be cited in the publication as: Escherichia coli (NCTC 14379)

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