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Ea.4

Ea.4

Catalogue No.

96073116

Cell Line Name

Ea.4

Cell Line Description

This cell line can be used to produce complex glycoproteins, and has been demonstrated as having more extensive N-glycosylation compared to other insect cell lines used for the expression of recombinant proteins. Ea.4 is susceptible to infection by Baculovirus, which can be used as a vector for recombinant protein synthesis.

Characteristics

Products

Complex glycoproteins

Tissue of Origin

Larvae

Morphology

Round

Karyotype

Not specified

Applications

Recombinant complex glycoprotein production, Baculovirus infection.

Disease

None Stated

Culture Conditions

Subculture Routine

Maintain cultures between 2x100,000 and 1x1,000,000 cells/ml. Doubling time 36 hours. Grow at 26°C. Freeze in 90% HI-FBS plus 10% glycerol.

Culture Medium

TiterHigh SF Insect Medium (Sigma I5408) (no supplements)

Growth Mode

Suspension

Additional Info

Depositor

Dr Kirit Patel, Research School of Biosciences, University of Kent, Canterbury

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Bio/Technology 1996;14:197

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: Ea.4 (ECACC 96073116).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.