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DBS-FRhL-2

DBS-FRhL-2

Catalogue No.

89101303

Cell Line Name

DBS-FRhL-2

Cell Line Description

A diploid cell line derived from normal male Rhesus monkey foetus. Susceptible to poliovirus types 1-3, Coxsackie A9, parainfluenza 3, rubella and is non-tumourigenic in animals.

General Info

Species

Monkey

Other Collection No.

ATCC CCL 160

Release Conditions

Restricted - CITES (Convention on the International Trade in Endangered Species of Wild Fauna and Flora) licence required for all orders outside the United Kingdom

Characteristics

Tissue of Origin

Lung

Karyotype

2n = 42, diploid no structural abnormalities observed

Applications

Virus studies

Disease

None Stated

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.05% trypsin; 5% CO₂; 37°C. Finite lifespan. Cell line has been shown to undergo 51 passages or 74 population doublings.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

In Vitro 1973;8:323

Bibliography

Not specified

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: DBS-FRhL-2 (ECACC 89101303).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.