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Clostridioides difficile

Clostridioides difficile

Catalogue No.

NCTC 14385

Current Name

Clostridioides difficile

Type Strain

Reference Strain

Original Strain Reference


Culture Conditions

Growth Conditions on Solid Media

Blood agar or fastidious anaerobe agar, 37°C, AnO₂, 24 - 48 hours

Biological and Genomic Info

Other Typing Conditions

PCR Ribotype 020, MLST type 002

Strain Data

Carries the 7-kb pCD-METRO plasmid / Phenotypically resistant to metronidazole / Carries an uncharacterised pCD6-family plasmid / At the request of the depositor, this strain is to be used for research purposes only, and not any commercial purpose, application or process.

Additional Info

Accession Date



Dr. Wiep Klaas Smits (Leiden University Medical Center, Dept. of Medical Microbiology, Leiden, The Netherlands)

Isolated From

Human with symptomatic C. difficile infection, faecal material. Leiden, The Netherlands. 03/02/2016.


Accessioned into the NCTC < Dr. Wiep Klass Smiths < Isolated by C�line Harmanus (Leiden University Medical Center, Leiden, The Netherlands)



Boekhoud IM, Hornung BVH, Sevilla E, et al. Plasmid-mediated metronidazole resistance in Clostridioides difficile. Nat Commun. 2020;11(1):598. Published 2020 Jan 30.

Available Formats

  • Ampoule (Bacteria)

If use of this culture results in a scientific publication, it should be cited in the publication as: Clostridioides difficile (NCTC 14385)

It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.