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UK Health Security Agency

CHSE-E

CHSE-E

Catalogue No.

20090901

Cell Line Name

CHSE-E

Cell Line Description

CHSE-E is a clonal cell line that permanently expresses a monomeric form of the Enhanced Green Fluorescent Protein (mEGFP) and is resistant to G418 (Geneticin).

This genetically modified cell line was derived from CHSE-214 cell line (Fish: Chinook Salmon (Oncorhynchus tshawytscha)).

Characteristics

Products

Permanently expresses a monomeric form of the Enhanced Green Fluorescent Protein (mEGFP)

Tissue of Origin

Embryo

Morphology

Pleomorphic

Applications

Susceptible to a wide range of fish viruses and, in many instances, replicates high titres.

Disease

No associated disease stated

Culture Conditions

Cell Type

Embryonic

Subculture Routine

Culture cells in the dark e.g. wrap vessel in foil.

At resuscitation, cells should be seeded at the higher end of the seeding density recommended: 4-5 x 10e4 cells/cm2 and culture for at least a week with 1-2 media changes.

Cells are slow growing but should establish themselves within 7-10 days.

Split sub-confluent cultures (70-80%) seeding cells at 1-4 x 10e4 cells/cm2 using 0.05% trypsin or trypsin/EDTA.

Cells should easily detach after 20-25 minutes at room temperature with gentle tapping.

Incubate at 5% CO2 and 21-22°C.

Freeze cells in 5% DMSO + 95% Foetal Bovine Serum (FBS).

Culture Medium

EMEM (EBSS) + 2mM L-Glutamine + 1% NEAA + 10% Foetal Bovine Serum (FBS) + 500µg/mL Genetecin (G418)

Growth Mode

Adherent

Additional Info

Depositor

Depositor: The Aquatic and Fish Health, Marine Scotland, Aberdeen, AB11 9DB, Scotland Originator: Dr Bertrand Collet Unite du Virologie et Immunologie Moleculaires, Institut National de la Recherche Agronomique (INRAE), Universite paris-Saclay, Jouy-en-Josas, France

Country of Origin

France

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Genetic Modification

This clonal, permanently expressing a monomeric form of the Enhanced Green Fluorescent Protein (mEGFP) and is resistant to G418. The plasmid pmEGFP-N1 confers resistance to Geneticin (G418) 500µg/ml

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Dehler CE, Boudinot P, Martin SA, Collet B. Development of an Efficient Genome Editing Method by CRISPR/Cas9 in a Fish Cell Line. Mar Biotechnol (NY). 2016 Aug;18(4):449-52. doi: 10.1007/s10126-016-9708-6. PMID: 27236514

Lester K, Hall M, Urquhart K, Gahlawat S, Collet B. Development of an in vitro system to measure the sensitivity to the antiviral Mx protein of fish viruses. J Virol Methods. 2012 Jun;182(1-2):1-8. doi: 10.1016/j.jviromet.2012.01.014. PMID: 22405879

Bibliography

Fryer JL, Yusha A, Pilcher KS. The in vitro cultivation of tissue and cells of Pacific salmon and steelhead trout. Ann N Y Acad Sci. 1965 Aug 10;126(1):566-86. doi: 10.1111/j.1749-6632.1965.tb14303.x. PMID: 5327928

Lannan, C.N., Winton, J.R. & Fryer, J.L. Fish cell lines: Establishment and characterization of nine cell lines from salmonids. In Vitro 20, 671–676 (1984). doi.org/10.1007/BF02618871

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: CHSE-E (ECACC 20090901).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

 

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.