skip to main content

BO0939

BO0939

Catalogue No.

94042659

Cell Line Name

BO0939

Cell Line Description

An Epstein-Barr virus (EBV) transformed lymphoblastoid cell line.

Characteristics

Tissue of Origin

Blood

Donor Sex

Male

Donor Age

14

Disorder

Fragile X Syndrome; Deletion

Morphology

Spherical

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml; 5% CO₂, 37°C

Culture Medium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). Once a growing culture is established the FBS concentration can be reduced to 10%.

Growth Mode

Suspension

Additional Info

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Genetic Modification

This is an Epstein-Barr Virus (EBV) transformed cell line

Hazard Group (ACDP)

2

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: BO0939 (ECACC 94042659)

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.