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Bacillus stearothermophilus

Bacillus stearothermophilus

Catalogue No.

NCTC 10003

Current Name

Bacillus stearothermophilus

Type Strain

Reference Strain

Original Strain Reference


Culture Conditions

Growth Conditions on Solid Media

Nutrient agar, O₂

Biological and Genomic Info

Gram Stain Result



D-xylose: -


Nitric acid: +

Enzyme Tests

Catalase: -

Phenylalanone aminotransferase: -

Strain Data

J. C. Kelsey, LSHTM in 1957 / Steam sterilization control / Kelsey, J. C. (1961) J. clin. Path. 14, 313

Colony Appearance

Smooth, Shiny

Additional Info

Accession Date






Previous Catalogue Name

Bacillus stearothermophilus



KELSEY J C 1961 J CLIN PATH 14 313

Extended Bibliography

Manachini,P.L, Craveri,A, Guicciardi,A., (1968), Composizione in basi dell acido desossiribonucleico di forme mesofile termofacoltative e termofile dell genere Bacillus., Ann. Microbiol. Enzimol., 1 (0), Gas-Sterilisatoren, Bio-Indikatoren zur Prüfung auf Wirksamkeit. DIN 58948, Teil 4. Kelsey,J.C., (1961), J. Clin. Pathol., 313 Spicher,G, Peters,J, Borchers,U., (1996), Bacillus subtilis and Bacillus staerothermophilus as test organisms or bioindicators: the effect of carrier properties on the resistance to steam., Hyg. Med., 190-195

Available Formats

  • Ampoule (Bacteria)

If use of this culture results in a scientific publication, it should be cited in the publication as: Bacillus stearothermophilus (NCTC 10003)

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