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Catalogue No.


Cell Line Name


Cell Line Description

The cell line was spontaneously derived from the porcine thyroid cell line PORTHOS (ECACC catalogue no. 93122323) after withdrawal of thyroid stimulating hormone (TSH). The cells form follicle-like structures, have a high growth rate, a high glucose consumption and are TSH independent for growth. The expression of plasminogen activators has been reported and found to be higher in ATHOS cells compared to PORTHOS cells.



Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone


Thyroglobulin, plasminogen activator (u-PA)

Tissue of Origin

Thyroid gland


Not specified


Mechanism of thyroid follicle reconstruction


None Stated

Culture Conditions

Cell Type


Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x100,000 cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO₂; 37°C.

Culture Medium

Click:RPMI (1:1) + 10µg/ml Insulin + 10nM Hydrocortisone + 5µg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).

Growth Mode


Additional Info


Dr G Fayet/Dr S Hovsepian, Laboratoire de Biochimie Medicale, Faculte de Medecine, Marseille, F-13385

Country of Origin


Hazard Group (ACDP)

Hazard Group (ACDP) 2



Cell Mol Biol 1993;39:427; Horm Metab Res 1993;25:238

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: ATHOS (ECACC 93122325).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.