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293TGPRT+R1

293TGPRT+R1

Catalogue No.

04072121

Cell Line Name

293TGPRT+R1

Cell Line Description

293T cells serially transfected with wild-type HIV Gag-Pol sequences expressed from a Murine Leukaemia Virus (MLV) vector; HIV tat sequences expressed from a MLV vector; HIV rev sequence expressed from a MLV vector and extra wild-type HIV-rev expressed from a MLV vector. These cells stably express wild-type HIV Gag-Pol, HIV tat and HIV rev sequences, as well as extra wild-type HIV rev. These cells serve as a packaging cell line for HIV based vectors.293T cells serially transfected with wild-type HIV Gag-Pol sequences expressed from a Murine Leukaemia Virus (MLV) vector; HIV tat sequences expressed from a MLV vector; HIV rev sequence expressed from a MLV vector and extra wild-type HIV-rev expressed from a MLV vector. These cells stably express wild-type HIV Gag-Pol, HIV tat and HIV rev sequences, as well as extra wild-type HIV rev. These cells serve as a packaging cell line for HIV based vectors.

General Info

Species

Human

Release Conditions

Restricted – all customers must complete the specific depositor MTA.

Characteristics

Tissue of Origin

Kidney (embryo)

Karyotype

Not specified

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 3-5x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 10% CO₂; 37°C. Cells may take up to 7 days to recover after resuscitation. If very few cells survive after 48 hours, it is advisable to wait another 2-7 days. During this time changing the media should be avoided as cells that are less than 10% confluent are extremely sensitive to any form of shock. These cells are more sensitive to pH and temperature shock than normal 293T cells, it is advisable to pre-warm media at 37°C before use. Cells detach easily at room temperature or during transit, therefore growing cultures may be received with cells in suspension. These cells must be frozen in glycerol not DMSO.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Professor Mary Collins, Department of Immunology and Molecular Pathology, University College London

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Ikeda Y et. al., Nat. Biotechnol. (2003) May: 21(5): 569-572

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: 293TGPRT+R1 (ECACC 04072121).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.