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1411H

1411H

Catalogue No.

06011805

Cell Line Name

1411H

Cell Line Description

A non seminomatous germ cell carcinoma derived cell line. A yolk sac carcinoma cell line derived from a testicular teratomacarcinoma. This cell line is tumourigenic in nude mice.

Characteristics

Tissue of Origin

Germ cell tumour

Karyotype

Not specified

Disease

Carcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 10% CO₂; 37°C.

Culture Medium

DMEM (High Glucose) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). No sodium pyruvate.

Growth Mode

Adherent

Additional Info

Depositor

Professor Peter Andrews, Department of Biomedical Sciences, Centre for Stem Cell Biology, The University of Sheffield, UK.

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Vogelzang et al., 1985. A human embryonal-yolk sac carcinoma model system in athymic mice. Cancer 55:2584-2593.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: 1411H (ECACC 06011805).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.