Cell culture reagents
The following list is provided to aid acquisition of reagents suitable for the culture of ECACC cell lines.
Please note there are other suppliers of these reagents; we are unable to endorse any specific manufacturer or supplier.
|
Catalogue no. |
Brand |
Product |
Description |
|---|---|---|---|
|
A1720 |
Sigma |
G418 |
G 418 disulfate salt |
|
A5006 |
Sigma |
L-Arginine |
L-Arginine |
|
B1522 |
Sigma |
BME |
Basal Medium Eagle |
|
B9285 |
Sigma |
Budr |
Bromo-deoxy Uridine |
|
C2639 |
Sigma |
Freeze Media SF |
Cell Freezing Medium – Serum free |
|
C3867 |
Sigma |
Collagen Type I |
Collagen Type I solution from rat tail |
|
C5467 |
Sigma |
CHO PF Media |
EX-CELL® ACF CHO Medium - Animal Component Free |
|
C5533 |
Sigma |
Collagen Type IV |
Collagen Type IV (from human placenta) |
|
C8919 |
Sigma |
Collagen Type I |
Collagen Type I (from calf skin) |
|
D2650 |
Sigma |
DMSO |
Dimethyl Sulphoxide (Hybri-Max™) |
|
D5796 |
Sigma |
DMEM |
DMEM, Dulbecco’s Modified Eagle’s Medium |
|
D8062 |
Sigma |
DMEM:F12 (1:1) |
DMEM:Nutrient Mix F12 Ham (1:1) ratio mix |
|
D8537 |
Sigma |
PBS |
Phosphate Buffered Saline |
|
F2442 |
Sigma |
FBS / FCS |
Foetal Bovine Serum / Foetal Calf Serum (USA Origin) |
|
F4135 |
Sigma |
FBS HI |
Foetal Bovine Serum – Heat Inactivated (USA Origin) |
|
F4759 |
Sigma |
Fibronectin |
Fibronectin |
|
21475025 |
Gibco |
Fischer’s Medium |
Fischer’s Medium |
|
F0855 |
Biochrom |
Coon’s F12 |
Nutrient Mixture F-12 Ham Coon’s Modification |
|
G1890 |
Sigma |
Gelatin |
Gelatin from Porcine skin |
|
G2025 |
Sigma |
Glycerol |
Glycerol |
|
G5154 |
Sigma |
GMEM |
Glasgow Minimum Essential Medium |
|
G7513 |
Sigma |
Glutamine |
L-Glutamine |
|
G9391 |
Sigma |
Gelatin |
Gelatin from Bovine skin |
|
H0137 |
Sigma |
HT |
HT media supplement (50x) Hybri-Max™ |
|
H0262 |
Sigma |
HAT |
HAT media supplement (50x) Hybri-Max™ |
|
H0887 |
Sigma |
HEPES |
HEPES solution |
|
H1270 |
Sigma |
HS |
Horse Serum |
|
H6909 |
Sigma |
Hydrocortisone |
Hydrocortisone solution |
|
H8142 |
Sigma |
HES |
Hybridoma Enhancing Supplement |
|
I0516 |
Sigma |
Insulin |
Insulin solution from Bovine pancreas. |
|
I3146 |
Sigma |
ITS |
Insulin – Transferrin – Selenium |
|
I3390 |
Sigma |
IDMEM |
IMDM / Iscove’s Modified Dulbecco’s Medium |
|
I7908 |
Sigma |
IL-2 |
Interleukin-2 human |
|
I9278 |
Sigma |
Insulin |
Insulin Solution - Human |
|
L5158 |
Sigma |
LIF |
Leukemia Inhibitory Factor (from mouse) |
|
L5520 |
Sigma |
L15 |
L-15 Medium (Leibovitz) |
|
L9010 |
Sigma |
LEH |
Lactalbumin Enzymatic Hydrolysate |
|
M2154 |
Sigma |
M199 |
Medium 199 |
|
M4526 |
Sigma |
ALPHA MEM |
Minimum Essential Medium Eagle’s – Alpha Modification |
|
M4655 |
Sigma |
EMEM (EBSS) |
Minimum Essential Medium Eagle’s (Earle’s Balanced Salt Solution) |
|
M5775 |
Sigma |
EMEM (HBSS) |
Minimum Essential Medium Eagle’s (Hank’s Balanced Salt Solution) |
|
M6250 |
Sigma |
2ME |
2 – Mercaptoethanol |
|
M6395 |
Sigma |
MCDB 105 |
MCDB 105 Medium |
|
M6895 |
Sigma |
VITS (MEM) |
MEM Vitamins |
|
M7145 |
Sigma |
NEAA |
Non Essential Amino Acids |
|
M8028 |
Sigma |
JMEM |
Minimum Essential Medium Eagle’s - Joklik Modification |
|
M8167 |
Sigma |
SMEM |
Minimum Essential Medium Eagle’s - Spinner Modification |
|
M8403 |
Sigma |
McCoy’s 5A |
McCoy’s 5A Medium Modified |
|
N1140 |
Sigma |
NCTC 109 |
NCTC 109 Medium |
|
N3520 |
Sigma |
Kaighn’s F12 |
Nutrient Mixture F-12 Ham Kaighn’s Modification |
|
N4637 |
Sigma |
NBCS |
New Born Calf Serum |
|
N6013 |
Sigma |
Ham’s F10 |
Nutrient Mixture F-10 Ham’s |
|
N6658 |
Sigma |
Ham’s F12 |
Nutrient Mixture F-12 Ham’s |
|
P8139 |
Sigma |
PMA |
Phorbol Myristate Acetate |
|
R7256 |
Sigma |
VITS (RPMI) |
RPMI Vitamins |
|
R8758 |
Sigma |
RPMI 1640 |
Roswell Park Memorial Institute |
|
S8636 |
Sigma |
NaP |
Sodium Pyruvate |
|
S8761 |
Sigma |
NaHCO3 |
Sodium Bicarbonate |
|
S9895 |
Sigma |
SDM |
Schneider’s Drosophila Medium |
|
T3160 |
Sigma |
TC-100 |
TC-100 Insect Medium |
|
25300 |
Sigma |
Trypsin/EDTA |
0.05% Trypsin-EDTA, phenol red |
|
T6414 |
Gibco |
Trypsin Inhibitor |
(Soybean) Trypsin Inhibitor |
|
T8154 |
Sigma |
Trypan Blue |
Trypan Blue |
|
T8159 |
Sigma |
TPB |
Tryptose Phosphate Broth |
|
W1625 |
Sigma |
WMB 752/1 |
Waymouth Medium MB 752/1 |
Last updated: April 2021