DNA extraction from whole blood or cultured cell lines
In ECACC, high quality DNA is extracted using automated high throughout systems. DNA is qualified for purity, size and integrity and is authenticated against source material using STR-PCR profiling to verify sample fidelity.
DNA extraction from cell lines
High quality genomic DNA can be prepared from your own cell lines deposited and stored at ECACC, in quantities ranging from a few micrograms, to milligram quantities, depending upon your needs (see below). In addition, DNA can be prepared from any cell line from ECACC's collections, should you require other than the standard quantities available.
|
Cell pellet size |
Approximate DNA yield expected |
|---|---|
|
1 – 1.5 x 107 |
40 – 60micrograms |
|
1.6 - 2 x 107 |
80 – 120micrograms |
|
3 x 107 |
120 – 180micrograms |
|
4 x 107 |
160 – 240micrograms |
|
5 x 107 |
200 – 300micrograms |
Standard Cell Culture Volumes & Approximate DNA Yields from Lymphoblastoid cell lines
DNA extraction from whole blood
DNA can be extracted from whole blood, fresh or frozen, using the latest automated technologies. The service is offered for blood volumes up to 10ml. The final DNA can be supplied in a variety of formats, both plates and tubes, according to your specifications. Blood for whole blood extraction should be sent in PLASTIC tubes not glass.