|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||TOU (TOU I-2)|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: TOU (TOU I-2) (ECACC 93093001)|
|Keywords:||Human B lymphocyte, EBV transformed|
|Cell Line Description:||Peripheral blood lymphocytes from a healthy female Tunisian immortalised by EBV transformation. The cell line contains the following deletions, in the human immunoglobulin heavy locus (IGH on chromosome 14 at 14q32.33), of 6 and 3 IGHC genes, respectively: on one chromosome 14, deletion I (del G1-EP1-A1-GP-G2-G4) and on the other chromosome 14, deletion II (del EP1-A1-GP). The Gm-Am genotype is Gm5*; - ; - A2m2/Gm21*;17,1; .. A2m2 (Gmb*; - ; - A2m2/Gmg*;za;.. A2m2). Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-). Only one allotype is defined on the gamma 2 chain and may or may not be present; (..) indicates the absence of this allotype. An Epstein-Barr-transformed lymphoblastoid cell line is available, from one son with identical deletions I and II, as ‘MOH (TOU II-3)’ (Catalogue No.: 93093002) and, from one son homozygous for deletion I, as ‘EZZ (TOU II-4)’ (Catalogue No.: 93093003).|
|Tissue of Origin:||blood|
|GMO Status:||Genetically Modified Organism Class 1 (GMO1)|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||Maintain cultures at 5-9x100,000 cells/ml; 5% CO2; 37°C.|
|Culture Medium:||RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).|
|Depositor:||Prof M-P Lefranc and Prof G Lefranc, Laboratoire d'ImmunoGénétique Moléculaire (LIGM), Université de Montpellier|
|References:||Lefranc M-P, Lefranc G, de Lange G, Out TA, van den Broek PJ, van Nieuwkoop J, Radl J, Helal AN, Chaabani H, van Loghem E, Rabbitts TH. 1983 Instability of the human immunoglobulin heavy chain constant region locus indicated by different inherited chromosomal deletions. Mol Biol Med. 1(2):207-17. PMID: 6438434|
|Additional Bibliography:||Lefranc M-P, Lefranc G. 1987 Human immunoglobulin heavy-chain multigene deletions in healthy individuals. FEBS Letters 213(2):231-7. PMID: 3104087|
|Patents:||None specified by Depositor|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Please confirm your country of origin from the list below.