|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||FM-3|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: FM-3 (ECACC 13012407)|
|Keywords:||Human melanoma, metastasis|
|Cell Line Description:||FM-3 (ESTDAB-007) was established from a metastatic tumour of a 70-year old female patient. The FM-3 cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commissions fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB (http://www.ebi.ac.uk/ipd/estdab) contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. This information is appended below. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation. Robinson J, Waller MJ, Fail SC, Marsh SG. 2006 The IMGT/HLA and IPD databases. Hum Mutat 27(12):11929.PMID: 16944494. Pawelec G, Marsh SG. 2006 ESTDAB: a collection of immunologically characterised melanoma cell lines and searchable databank. Cancer. Immunol Immunother. 55(6):623-7.PMID: 16421722. Robinson J, Roberts CH, Dodi IA, Madrigal JA, Pawelec G, Wedel L, Marsh SG.2009 The European searchable tumour line database. Cancer. Immunol Immunother. 58(9):1501-6. PMID: 19172270.|
|Tissue of Origin:||Skin|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||Split sub-confluent cultures (70-80%) seed new cultures at 1.5-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density 11x10,000 cells/cm² .|
|Culture Medium:||RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).|
|Depositor:||J Zeuthen, Department of Tumour Cell Biology, Division of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, Bldg 7.1, DK-2100 Copenhagen Denmark.|
|References:||Kirkin AF, Petersen TR, Olsen AC, Li L, thor Straten P, Zeuthen J.1995 Generation of human-melanoma-specific T lymphocyte clones defining novel cytolytic targets with panels of newly established melanoma cell lines. Cancer Immunol Immunother. 41(2):71-81. PMID: 7656272.|
|Additional Bibliography:||Halder T, Pawelec G, Kirkin AF, Zeuthen J, Meyer HE, Kun L, Kalbacher H. 1977 Isolation of novel HLA-DR restricted potential tumor-associated antigens from the melanoma cell line FM3. Cancer Res.; 57(15):3238-44. PMID: 9242455. Olsen AC, Fossum B, Kirkin AF, Zeuthen J, Gaudernack G. 1995 A human melanoma cell line, recognized by both HLA class I and class II restricted T cells, is capable of initiating both primary and secondary immune responses. Scand J Immunol. 41(4):357-64. PMID: 7899823. Zeuthen J, Dzhandzhugazyan K, Hansen MR, Kirkin AF. 1998 The immunogenic properties of human melanomas and melanoma-associated antigens recognized by cytotoxic T lymphocytes. Bratisl Lek Listy. 99(8-9):426-34. PMID: 9810766.|
|Patents:||None specified by Depositor|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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