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L929/A

L929/A

Catalogue No.

14112101

Cell Line Name

L929/A

Cell Line Description

This Adriamycin-resistant cell line has been developed by exposure of the parent L929 murine fibroblast cell line (ECACC catalogue number 85011425) to increasing concentrations of doxorubicin in-vitro. L929/A cells can be used in the development of novel anti-cancer treatments. Resistance can be circumvented by modulating agents such as verapamil and quinine. The parent cell line L929 was derived from normal subcutaneous areolar adipose tissue.

Characteristics

Tissue of Origin

Adipose

Karyotype

Not specified

Disease

None Stated

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures vessels at 1-2 x 10⁴ cells /cm². Cultures incubated in a humidified 5% CO₂/95% air incubator at 37°C.

Culture Medium

RPMI 1640 + 10% Foetal Bovine Serum (FBS) + 2mM L-Glutamine + 0.086uM Doxorubicin hydrochloride.

Growth Mode

Adherent

Additional Info

Depositor

Trust Diya, Staffordshire University, Science Centre, Leek Road, Stoke-on-Trent, ST4 2DE

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Cadagan D, Merry S. Circumvention of inherent or acquired cytotoxic drug resistance in vitro using combinations of modulating agents. Anticancer Res. 2013 Oct; 33 (10):4381-7.

Bibliography

Earle WR at.al. Production of malignancy in vitro, IV. The mouse fibroblast cell cultures band changes seen in living cells. J Natl Cancer Inst. 1943, 4:165-212.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: L929/A (ECACC 14112101).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.