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Catalogue No.


Cell Line Name


Cell Line Description

The T47D/S5 cell line is the control for the fulvestrant-resistant T47D/182R-1 and T47D/182R-2 cell lines.

General Info



Unique to ECACC


Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.



T47DS5 cells express ER alpha and progesterone receptor.

Tissue of Origin



Polygonal epithelia

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,13
D3S1358: 15,17
D5S818: 12
D7S820: 11
D8S1179: 13
D13S317: 12
D16S539: 10
D18S51: 17
D21S11: 28,31
FGA: 23
Penta D: 10,12
Penta E: 7,14
TH01: 6
TPOX: 11
vWA: 14


The T47D/S5 Cell line is the control for the fulvestrant resistant lines T47D/182R-1 and T47D/182R-2 cell lines. Anti-oestrogen resistance is a major problem in breast cancer treatment. Therefore, the search for new therapeutic targets and biomarkers for anti-oestrogen resistance is crucial.


Pleural Effusion of a Ductal Carcinoma

Culture Conditions

Cell Type


Subculture Routine

Split sub-confluent cultures at 70-80% confluency. Subculture cells using 0.05% trypsin/EDTA solution and soya bean trypsin inhibitor or TrypLE Express™ (1X), without phenol red, and wash with phosphate- buffered saline (PBS). Pellet cells by centrifugation and re-suspend in growth medium. Initiate the culture at a dilution of approximately 1:3 to 1:6 i.e. seeding at 2-4 x 10⁵ cells per cm². Incubate cultures in 5% CO₂ in air at 37°C. Recommend media changes every 2-3 days. Cells can be cryopreserved in either a mixture of cell line conditioned medium (45%) and fresh serum free-medium (45%) with 10% dimethyl sulphoxide (DMSO) or 90% Foetal Bovine Serum (FBS) and 10% dimethyl sulphoxide (DMSO). It is recommended that upon resuscitation the cells are washed in phosphate- buffered saline (PBS) or serum-free medium, pelleted by centrifugation and re-suspended in growth medium prior to initiation of culture.

Culture Medium

Phenol red free RPMI 1640 + 5% FCS + glutamax + 8µg Insulin/ml.

Growth Mode


Additional Info


Ximbio (formerly known as Cancer Research Technologies Limited)

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2



Larsen et al. 2015. PLoS One. 10(2):e0118346. PMID: 25706943.Larsen et al. 2015. BMC Cancer. 15:239. PMID: 25885472. Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.Thrane S, et al. Oncogene. 2015 Aug 6;34(32):4199-210. doi: 10.1038/onc.2014.351. Epub 2014 Nov 3.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: T47D/S5 (ECACC 16022511).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.