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HMT-3522 S1

HMT-3522 S1

Catalogue No.


Cell Line Name

HMT-3522 S1

Cell Line Description

HMT-3522 S1, also known as HMT-3522/wt, is a subline that has been derived from HMT-3522. The parent line was established from a benign breast tumour of a 48 year old woman and has undergone spontaneous malignant transformation. It was grown on collagen in serum-free media without antibiotics from explantation onwards. A mutation at codon 179 of the p53 gene was detected during establishment of the parent line.HMT-3522 S1 is a continuation of this line from passage 34 and is grown without collagen.It is near-diploid, but an evolution of karyotype has been observed by regular chromosomal analyses. The expression of cytokeratin 13, 14, 17 and 18 and vimentin has been reported. Expression of EGF-R, c-erb-B2, N-myc, c-ras-Ha1, c-ras-Ki2 and N-ras was shown to fluctuate slightly with passage number. The cells have tested negative for estrogen receptor, c-erbA1, c-erb-A2, int-2 or hst-1. During propagation growth factor requirements decreased simultaneously with amplification and overexpression of c-myc protooncogene. In a three-dimensional basement membrane culture assay the phenotypic characteristics of normal breast tissue in vivo are recapitulated. This cell line has remained non-tumorigenic for more than 400 passages. Publications using this cell line must refer to the original paper (Briand et al., In Vitro Cell Dev Biol 1987; 23:181). Any sublines created must include the name HMT-3522 in the designation, but a short name may be used in the same paper.Subline HMT-3522 S2 (ECACC catalogue no. 98102211) has been isolated by culturing S1 cells from passage 118 in serum-free media without epidermal growth factor (EGF). This cell line grows on collagen-treated flasks.The third subline, HMT-3522 T4-2 (ECACC catalogue no. 98102212) was established from S2 at passage 238 and is the only tumorigenic line in nude mice out of the 3 sublines. This cell line grows on collagen-treated flasks.




Tissue of Origin





Evolution of karyotype and growth factor requirements, model for premalignant breast epithelium and study of mechanism of malignant conversion



Culture Conditions

Cell Type


Subculture Routine

If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium and re-suspend cells, taking a sample of the cell suspension, e.g. 100µl, to count. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes.Split sub-confluent cultures using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells are slow to detach from the flask. Add the same volume of trypsin inhibitor as trypsin used, centrifuge and reseed cells at 2-4 x10, 000 cells/cm². Medium is serum-free, therefore trypsin inhibitor is essential. S1cells can be slow in spreading out after subculture and may take 2-3 days to attach. Good growth occurs 5-7 days after subculture.Cells should be frozen in (45% conditioned media: 45% fresh media) with 10% DMSO.

Culture Medium

DMEM / Ham's F12 (1:1) + 2mM Glutamine + 250ng/ml insulin + 10µg/ml transferrin +10⁻⁸M sodium selenite + 10⁻¹⁰M 17 β-estradiol + 0.5µg/ml hydrocortisone + 5µg/ml ovine prolactin + 10ng/ml EGF

Growth Mode


Additional Info


Dr P Briand, Department of Tumour Endocrinology, Institute of Cancer Biology, Danish Cancer Society, Copenhagen, Denmark

Country of Origin


Hazard Group (ACDP)

Hazard Group (ACDP) 2



In Vitro Cell Dev Biol 1987; 23:181;Cancer Res 1992;52:1210; Cancer Res 1996;56:2039; J Cell Biol 1997; 137: 231; Genes Chromosom Cancer 1997; 20: 30; Nat Biotechnol 1997; 15: 517

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: HMT-3522 S1 (ECACC 98102210).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.