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Catalogue No.


Cell Line Name


Cell Line Description

The HEK293-A GFP-WIPI1 cell line can be used to study autophagy by light microscopy. It is also useful for high throughput microscopy screens. This is a stable cell line expressing GFP-tagged human WIPI1 in background of HEK293-A cell line. The parental line HEK293-A (also known as HEK293A) cells were transfected with GFP-WIPI1 then selected with G418, and stable, amino acid responsive clones were selected. The HEK293-A GFP-WIPI1 cell line is also listed in the scientific literature as “HEK293A GFP-WIPI1". They are derived from the parental cell line HEK293-A.

General Info



Unique to ECACC


Also Known As


Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.


Tissue of Origin

Embryonic Kidney


Adherent polygonal epithelium

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 12
D3S1358: 15,17
D5S818: 8
D7S820: 11
D8S1179: 12,14
D13S317: 12,14
D16S539: 9,13
D18S51: 17
D21S11: 30.2
FGA: 23
Penta D: 9
Penta E: 7,15
TH01: 9,9.3
TPOX: 11
vWA: 16,19


Apoptosis and Programmed Cell Death, Cancer, Cell Signaling & Signal Transduction, Cellular Transport, Drug Discovery & Development, Metabolism


None Stated

Culture Conditions

Cell Type


Subculture Routine

Trypsinise cultures at 80% confluence with 0.05% Trypsin/EDTA. Seed flasks at 2x10⁴ cells/cm². Cultures must be incubated in a humidified 10% CO₂/90% air incubator at 37°C.

Culture Medium

Dulbecco's Modified Eagle's Medium, high glucose (DMEM) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode


Additional Info


Ximbio (formerly known as Cancer Research Technologies Limited)

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Genetic Modification

HEK293 cells stably expressing GFP-WIPI1 were established by transient transfection with pEGFP-constructs followed by selection with G418 (800 µg/ml) and cloned by limiting dilution and selected for low level expression by FACs sorting. Cells maintained in the presence of G418 (400 µg/ml).

Hazard Group (ACDP)

Hazard Group (ACDP) 2



Dooley HC, Razi M, Polson HE, Girardin, Wilson MI, Tooze SA. WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1. Mol Cell. 2014 Jul 17;55(2):238-52. doi: 10.1016/j.molcel.2014.05.021. Epub 2014 Jun 19. PMCID: PMC4104028, PMID: 24954904

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: HEK293-A GFP-WIPI1 (ECACC 17100533).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.