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Catalogue No.


Cell Line Name


Cell Line Description

Adherent keratinocyte cell line derived from a lymph node metastasis squamous cell carcinoma (SSC) of the larynx. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are tumourigenic in athymic mice. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

General Info



Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.


Tissue of Origin


DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,12
D5S818: 12
D7S820: 9
D13S317: 8,13
D16S539: 12,14
TH01: 7
TPOX: 9,12
vWA: 17,20




Squamous Cell Carcinoma

Culture Conditions

Cell Type


Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 using 0.05% trypsin/EDTA; 8% CO₂; 37°C. Suggested seeding density 4 x 10,000 cells/cm². Use of conditioned medium at a ratio of 1:1 with fresh medium can improve cell condition. Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 Swiss Albino cells (ECACC Catalogue number: 85022108). Feeder layers are prepared in the flasks at least 24 hours in advance of being required. Where frozen stocks of treated 3T3 Swiss Albino cells have been prepared, an ampoule is thawed in 37°C water bath and the contents quickly transferred to a 15ml centrifuge tube. DMEM medium is added drop wise to 5ml. Cells are centrifuged at 150 x g for 5 minutes at Room Temperature. Cells are resuspended in 5ml of medium. Cells are counted and added to flasks containing the correct BICR growth medium at 1-3 x 10⁴ cells/cm².

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 µg/ml Hydrocortisone.

Growth Mode


Additional Info


Prof. Kenneth Parkinson, Cancer Research Campaign Beatson Laboratories, Garscube Estate, Switchback Road, Glasgow G61 1BD

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2



Edington KG, Loughran OP, Berry IJ, Parkinson EK 1995 Cellular immortality: a late event in the progression of human squamous cell carcinoma of the head and neck associated with p53 alteration and a high frequency of allele loss. Mol Carcinog. 13(4):254-65 PMID: 7646764.


Loughran O, Clark LJ, Bond J, Baker A, Berry IJ, Edington KG, Ly IS, Simmons R, Haw R, Black DM, Newbold RF, Parkinson EK 1997 Evidence for the inactivation of multiple replicative lifespan genes in immortal human squamous cell carcinoma keratinocytes Oncogene. 14(16):1955-64 PMID: 9150362. Munro J, Stott FJ, Vousden KH, Peters G, Parkinson EK 1999 Role of the alternative INK4A proteins in human keratinocyte senescence: evidence for the specific inactivation of p16INK4A upon immortalization.Cancer Res. 59(11):2516-21 PMID: 10363964. Barretina J, et al., 2012 The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature. 483(7391):603-7. PMID: 22460905.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: BICR 18 (ECACC 06051601).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.